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KMID : 0614619920240030547
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Korean Journal of Gastroenterology
1992 Volume.24 No. 3 p.547 ~ p.553
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The Relationship of the Activation of ras Oncogene to Hepatitis B Virus Gene Expression in Human Hepatocellular Carcinoma
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Abstract
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substantial epidemiological and molecular biological evidence shows that hepatitis B virus is the most common etiologic agent of human hepatocellular carcinoma. Several studies have revealed increased expression of ras oncogene in hepatocellular
carcinoma tissues both in experimental animal and in human. This study was performed to determine the direct relationship of the activation of ras oncogene in human HCC to the expression of HBV genome at the cellular level, using
immunocytochemical
analysis to detect HBsAg and ras p21 on the subsequent different tissue sections and immunocytochemical assay for the detection of ras p21 in combination with in situ hybridization for the detection of HBV DNA/RNA on the same tissue section. Of
the
twenty-two cases of HCC tissue sections studied, enhanced expression of ras p21 was observed in one HCC case (4.5%); the distribution of ras p21-expressing HCC cells was focal. HBsAg and HBV DNA/RNA were mnostly found in nontumorous hepatocytes
but
not
in ras p21-expressing HCC cells. These findings suggested that continued expression of HBsAg or the presence of HBV DNA/RNA is not necessary for the activation of ras proteins in a given human HCC cell. We conclude that ras oncogene is not
directly
activated by HBV gene expression.,
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KEYWORD
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